DNA

Part:BBa_K1732006

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)
Revision as of 06:37, 14 September 2015 by Donnalee (Talk | contribs)

pSB1C3-J23100-B0034-Firefly-B0015

J23100-B0034-Firefly-B0015

J23100 Promoter (BBa_J23100) with RBS (BBa_B0034), Firefly Luciferase Sequence (BBa_I712019), and B0015 Terminator (BBa_B0015).

Firefly Luciferase, also known as FLuc, is a luciferase from Photinus pyralis. It is a protein that emits light when reacted with the appropriate substrate luciferin. It is able to act as a report protein when linked to other promoters of proteins. The luminescence generated can also be used to measure the luminescence of standard luminometers and compare it to the measurements by DIY luminometers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 465
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 370
    Illegal AgeI site found at 1640
  • 1000
    COMPATIBLE WITH RFC[1000]



Example11.jpg

This sequence was codon optimized to allow maximal amounts of proteins to be created and extracted.

Kenneth.jpg

[edit]
Categories
Parameters
None