Coding

Part:BBa_K1595030:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-13)
Revision as of 04:19, 14 September 2015 by J.Chen (Talk | contribs)

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EDA linked to cel5a via GSG linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 641
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 1494
    Illegal AgeI site found at 1758
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1755


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Cel5a is a stable, detectable protein that can be used to determine expression of our plasmid.

EDA had an internal PstI cutsite that was removed by altering the DNA sequence.

Source

Synthesize by DNA 2.0

References

EDA: NCBI accession number P0A955

Cel5a: Pereira et al. Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima. Journal of Structural Biology. Vol 172(3). Dec 2010. 372-379.