![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K1595030:Design
Designed by: Jonlin Chen Group: iGEM15_Cornell (2015-09-13)
EDA linked to cel5a via GSG linker
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1965
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 641
Illegal NgoMIV site found at 1038
Illegal NgoMIV site found at 1494
Illegal AgeI site found at 1758 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1755
Design Notes
Use in conjunction with strain-expressing T7 polymerase.
cel5a is a stable, detectable protein that can be used to determine expression of our plasmid.
Source
Synthesize by DNA 2.0