Coding
NdeI+E0040

Part:BBa_K1819000:Design

Designed by: Lais Ribovski   Group: iGEM15_Brasil-USP   (2015-09-13)
Revision as of 20:34, 13 September 2015 by Laisribovski (Talk | contribs) (→‎Source)


GFP coding sequence with an N-terminal linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 674


Design Notes

As a design consideration we highlight that we chose to add the NdeI restriction site to a GFP coding sequence to avoid any additional amino acid residue between coding sequence and the fused N-terminal tag, then it should not have any scar.

Source

GFP coding sequence source is BBa_E0040.

References