Coding

Part:BBa_K1669004

Designed by: Nina Jerala   Group: iGEM15_Slovenia_HS   (2015-09-13)
Revision as of 19:51, 13 September 2015 by Registry (Talk | contribs)

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BdhB + His-tag

This part includes the BdhB gene from Clostridium acetobutylicum (NCBI: CAP0163) with a C-terminal His-tag, which has been codon optimized for expression in E. coli. This is the gene coding the butanol dehydrogenase enzyme, that is in Clostridium acetobutylicum responsible for conversion of butyryl-CoA to butanol. The sequence contains a C-terminal His-tag for purification of the protein using the immobilized-metal affinity chromatography and detected with the anti-His antibodyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1021


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Categories
Parameters
None