Part:BBa_K1632004
fim switch[default ON](wild-type)
Fim switch (wild-type) is derived from wild-type sequence. Fim switch (wild type) have sigma 70 promoter which is constitutively promoter. The promoter in the fim switch transcripts to the right. From the direction of transcription, the state is defined as ON.
Fim switch is inverted by two recombinase, FimB and FimE.The FimB protein inverts fim switch in the ON-to-OFF and the OFF-to-ON direction with approximately equal efficiencies. On the other hand, the FimE protein inverts fim switch predominantly in the ON-to-OFF direction.
We constructed fim switch[ON](wild-type)-rbs-gfp(BBa_K1632007) to characterize the function of this part, by inserting this promoter upstream of a GFP coding sequence.
In the fimB dependent fim switch state assay, we transformed fim switch[ON](wild-type)-rbs-gfp and pBAD/araC-fimB(BBa_K1632012)in the E.coli DH5alfa strain. We measured the fluorescence intensity of the cells induced by arabinose.
Similary,in the fimE dependent fim switch state assay, we transformed fim switch[ON](wild-type)-rbs-gfp and pBAD/araC-fimE(BBa_K1632013) in the E.coli DH5alfa strain.We measured the fluorescence intensity of the cells induced by arabinose.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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