Composite
Part:BBa_K1871002
Designed by: Broad Run High School iGEM Team Group: iGEM15_BroadRun-NorthernVA (2015-09-13)
Alpha amylase with mating factor alpha secretion
This is the alpha amylase from Bacillus amyloliquefaciens, flanked by Kozak sequence (Part BBa_K165002), mating factor alpha secretion sequence (Part BBa_K792002), and the ADH1 Terminator (Part BBa_K392003). This part is contained by the minimal cyc promoter (Part BBa_K105027).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1497
Illegal NgoMIV site found at 1512
Illegal AgeI site found at 1815 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//chassis/eukaryote/yeast
None |