Composite

Part:BBa_K1871002

Designed by: Broad Run High School iGEM Team   Group: iGEM15_BroadRun-NorthernVA   (2015-09-13)
Revision as of 18:53, 13 September 2015 by Registry (Talk | contribs)

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Alpha amylase with mating factor alpha secretion

This is the alpha amylase from Bacillus amyloliquefaciens, flanked by Kozak sequence (Part BBa_K165002), mating factor alpha secretion sequence (Part BBa_K792002), and the ADH1 Terminator (Part BBa_K392003). This part is contained by the minimal cyc promoter (Part BBa_K105027).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 1512
    Illegal AgeI site found at 1815
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None