Composite

Part:BBa_K1871001

Designed by: Broad Run High School iGEM Team   Group: iGEM15_BroadRun-NorthernVA   (2015-09-13)
Revision as of 18:42, 13 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Alpha amylase with native secretion

This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence (Part BBa_K165002) the ADH1 terminator (Part BBa_K392003) . Expression is controlled by the cyc yeast promoter (Part BBa_K105027).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1533
    Illegal NgoMIV site found at 1548
    Illegal AgeI site found at 1851
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None