RNA

Part:BBa_K1777003

Designed by: Xilin Jiang   Group: iGEM15_Fudan   (2015-09-13)
Revision as of 17:51, 13 September 2015 by Registry (Talk | contribs)

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miR-21 linear sponge

This part has 6 mir-21 binding site and is flanked by bate-globing intron1 splicing site. This parts can sequester mir-21. Add cyclizing device to the flanking region of this part, it can be involved into a back-splicing mechanism and become a circRNA sponge.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
    Illegal XhoI site found at 452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 423


[edit]
Categories
Parameters
None