Coding
Part:BBa_K1766008
Designed by: Pontus Höjer Group: iGEM15_Stockholm (2015-09-13)
EnvZ_V5 osmoregulatory histidine kinase from E.coli.
EnvZ is part of the EnvZ-OmpR two-component regulatory system for controlling osmotic pressure in E.coli. Structure includes a C-terminal V5-tag through a GGSSAAG linker. The part was used in a pRD400 backbone behind a lac-inducible promoter to study signaling as well as expression.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 316
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 316
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 316
Illegal BglII site found at 236 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 316
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 316
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |