Coding

Part:BBa_K1633008

Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei   Group: iGEM15_NJU-China   (2015-09-13)
Revision as of 08:13, 13 September 2015 by Registry (Talk | contribs)

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coding sequence to express nSMase 2 protein.

This part is a codon-optimized nSMase 2 gene CDS. When this part is inserted into pcDNA 3.1 vector and transfected into mammalian cells,it can express nSMase2 gene in mammalian cells and stimulate the secretion of exosomal siRNAs from cells.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 472
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1609
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 278
    Illegal BsaI.rc site found at 694
    Illegal BsaI.rc site found at 1600


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Categories
Parameters
None