Coding
Part:BBa_K1633008
Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei Group: iGEM15_NJU-China (2015-09-13)
coding sequence to express nSMase 2 protein.
This part is a codon-optimized nSMase 2 gene CDS. When this part is inserted into pcDNA 3.1 vector and transfected into mammalian cells,it can express nSMase2 gene in mammalian cells and stimulate the secretion of exosomal siRNAs from cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 472
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1609
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 278
Illegal BsaI.rc site found at 694
Illegal BsaI.rc site found at 1600
[edit]
Categories
Parameters
None |