RNA

Part:BBa_K1633007:Design

Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei   Group: iGEM15_NJU-China   (2015-09-13)
Revision as of 08:09, 13 September 2015 by Registry (Talk | contribs)

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GFP siRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed specific GFP siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene GFP to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.



Source

We designed the GFP 122 siRNA and ordered the part from a DNA synthesis company.


References