RNA
Part:BBa_K1633006:Design
Designed by: Chen Xi, Zhou Yu, Jiang Waner, Zhang Peng, Tian Chenfei Group: iGEM15_NJU-China (2015-09-13)
MOR siRNA-4 (siRNA for mouse Mu opioid receptor)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed specific MOR siRNAs based on a free software accessible online. This tool can find the best siRNA sequences on target gene MOR to insure the maximum gene-specificity and silencing efficacy. This tool also designs the pair of oligonucleotides needed to generate short hairpin RNAs (shRNAs) in the plasmid.
Source
We designed the MOR siRNA-4 and ordered the part from a DNA synthesis company.