Coding

Part:BBa_K1595020:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-09-12)
Revision as of 06:31, 13 September 2015 by J.Chen (Talk | contribs) (→‎References)


MBP with EcnB from Ralstonia pickettii.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1257
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1257
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1257
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 138


Design Notes

Use in conjunction with strain-expressing T7 polymerase.

Has internal PstI cutsite.

Source

Synthesized by DNA 2.0

References

EcnB: NCBI source number AGW91308 MBP: BBa_K407015