Translational_Unit

Part:BBa_K1631002

Designed by: Yuto Yamanaka   Group: iGEM15_UT-Tokyo   (2015-09-12)
Revision as of 04:17, 13 September 2015 by Yamanaka (Talk | contribs)

Translational unit of Colicin-E3

Colicin-E3 cleave 16SrRNA at a specific site. This activity is inhibited by immunity protein for Colicin-E3(BBa_). When lysis protein for Colicin-E3(BBa_) is co-expressed, Colicin-E3 is released to environment and kill other strain.

Colicins are type of bacteriocin produced by Escherichia coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1526
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 489
    Illegal AgeI site found at 241
    Illegal AgeI site found at 1507
    Illegal AgeI site found at 1612
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/celldeath
//function/cellsignalling
Parameters
None