Composite

Part:BBa_K1639000:Design

Designed by: Mustafa Yılmaz   Group: iGEM15_ATOMS-Turkiye   (2015-09-12)
Revision as of 20:13, 12 September 2015 by Mustafa26 (Talk | contribs) (→‎Design Notes)


Toehold-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 772


Design Notes

This part contains additional BamHI restriction site between Toehold and GFP for replacing a new gene to downstream of Toehold sequence.

Source

Wyss Institute for Biologically Inspired Engineering. Sequence is taken from supplementary data of article: 'Toehold Switches: De-Novo-Designed Regulators of Gene Expression'. (PMID: 25417166)

References