Composite

Part:BBa_K1714000:Design

Designed by: Hiroki ONO   Group: iGEM15_HokkaidoU_Japan   (2015-09-10)
Revision as of 07:40, 12 September 2015 by Mami (Talk | contribs) (→‎Source)


pBad/araC-RBS B0034-Ag43 thanatin as alpha domain-double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1468
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1750
    Illegal NgoMIV site found at 2260
    Illegal NgoMIV site found at 2281
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1413
    Illegal AgeI site found at 2020
    Illegal AgeI site found at 2434
    Illegal AgeI site found at 2676
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This part has 6 PstI sites so you shoud not digest this part by PstI.


Source

This part is derived from BBa_K759001 and it contains thanatin-Asp fragment that is synthesized by IDT.

testtest

References