Composite

Part:BBa_K1638003

Designed by: Jens Sivkćr Pettersen   Group: iGEM15_SDU-Denmark   (2015-05-09)
Revision as of 10:42, 11 September 2015 by Jpettersen (Talk | contribs)

T25 domain of cyaA from Bordetella pertussis (IPTG inducible)

TThe T25 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the conjugated proteins associates, T25 and T18 associates too. This leads to the catalysation of the conversion of ATP to cAMP. The rise in cyclic AMP can trigger the expression of genes by using a cAMP-induced promotor. This promotor could for example regulate the expression of a red flourescent protein (RFP). The presence of red-fluorescent cells can in turn be used to verify protein-protein interactions.

See BBa_K1638003 for the T18 domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 843
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None