Part:BBa_K1638005

T18 domain of cyaA from Bordetella pertussis (IPTG inducible)

The T18 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the conjugated proteins associates, T25 and T18 associates too. This leads to the catalysation of the conversion of ATP to cAMP. The rise in cyclic AMP can trigger the expression of genes by using a cAMP-induced promotor. This promotor could for example regulate the expression of a red flourescent protein (RFP). The presence of red-fluorescent cells can in turn be used to verify protein-protein interactions.

See BBa_K1638003 for the T25 domain.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 711
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 252
  Illegal NgoMIV site found at 662
  Illegal AgeI site found at 468
  
• 1000
  COMPATIBLE WITH RFC[1000]