Part:BBa_K1638005
T18 domain of cyaA from Bordetella pertussis (IPTG inducible)
The T18 domain of CyaA to be used in the bacterial two-hybrid system. The expression of the gene is under control of a lac operator and is induced by Isopropyl β-D-1-thiogalactopyranoside (IPTG). When protein-coding genes is suffixed to the T25 and T18 domains of the bacterial two-hybrid system, the interaction of these two proteins can be examined. If the conjugated proteins associates, T25 and T18 associates too. This leads to the catalysation of the conversion of ATP to cAMP. The rise in cyclic AMP can trigger the expression of genes by using a cAMP-induced promotor. This promotor could for example regulate the expression of a red flourescent protein (RFP). The presence of red-fluorescent cells can in turn be used to verify protein-protein interactions.
See BBa_K1638003 for the T25 domain.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
None |