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Part:BBa_K1627006
Device to demethylate caffeine
This part contains genes that allow for the demethylation of caffeine. The part contains a synthetic operon consisting of ndmA, ndmB, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of caffeine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of caffeine, a growth curve can be made that correlates culture growth with concentration of caffeine. Then if the strain is grown in a minimal media with an unknown concentration of caffeine, the growth can be compared to the growth curve to calculate the initial concentration of caffeine in the media.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1733
Illegal NheI site found at 1928 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2684
Illegal BglII site found at 3180
Illegal BglII site found at 3651
Illegal XhoI site found at 4792 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 5490
Illegal AgeI site found at 3319 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 983
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