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Part:BBa_K1627003
Device to demethylate theophylline
This part contains genes that allow for the demethylation of theophylline. The part contains a synthetic operon consisting of ndmA, ndmB, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.
This part can be used in a ∆guaB strain of E. coli to measure the amount of theophylline in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of theophylline, a growth curve can be made that correlates culture growth with concentration of theophylline. Then if the strain is grown in a minimal media with an unknown concentration of theophylline, the growth can be compared to the growth curve to calculate the initial concentration of theophylline in the media.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1733
Illegal NheI site found at 1928 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2296
Illegal BglII site found at 2767
Illegal XhoI site found at 3908 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4606
Illegal AgeI site found at 2435 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 983
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