Translational_Unit

Part:BBa_K1604000

Designed by: Silvia Galvan   Group: iGEM15_UNITN-Trento   (2015-07-08)
Revision as of 16:07, 10 September 2015 by Sil (Talk | contribs)

Strong RBS + Proteorhodopsin

Proteorhodopsin (PR) is a light-powered proton pump. It creates a proton motive force across the membrane that can subsequently power cellular processes, such as ATP synthesis.

Usage and Biology

We improved part BBa_K773002 of Caltech 2012 by adding a strong RBS. We placed BBa_K1604000 under an inducible promoter and we fully characterized proteorhodopsin.
To see the characterization results check the part BBa_K1604010.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 529
    Illegal XhoI site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 666
  • 1000
    COMPATIBLE WITH RFC[1000]



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Categories
Parameters
None