Composite

Part:BBa_K1604022

Designed by: Veronica Pinamonti   Group: iGEM15_UNITN-Trento   (2015-08-20)
Revision as of 15:41, 10 September 2015 by Cridelbianco (Talk | contribs)

araC-pBAD + β-carotene + J23100 + blh

This biobrick is obtained by the assembly of two parts K1604020 and K1604021. The composite part contains the five genes necessary for the retinal synthesis. The first part contains ctrEIBY under araC-pBAD, these four gene are responsive of beta-carotene production starting from FPP, a colurless precurse presents in E.coli. The second part contains blh under costitutive promoter. It encodes for the beta-carotene15-15'dioxygenase that catalizes the cleavege of a single molecule of beta-carotene into two molecules of retinal.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
    Illegal NheI site found at 32
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2041
    Illegal BamHI site found at 4082
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 247
    Illegal NgoMIV site found at 3618
    Illegal NgoMIV site found at 3748
    Illegal AgeI site found at 1876
    Illegal AgeI site found at 2833
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1858


[edit]
Categories
Parameters
n/aaraC-pBAD + β-carotene + J23100 + blh