Translational_Unit

Part:BBa_K1641015

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)
Revision as of 14:02, 10 September 2015 by Registry (Talk | contribs)

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Up-side-down RBS::mcherry-ssra with FRT recognition sites

This is a special RBS::mcherry-ssra inverted into up-side-down form, with two FRT sites at the upstream and downstream of the gene. FRT can be recognized and the middle sequence can be restored by invertase Flp. Ssra-tag in this sequence is moderately fast type (BBa_M0052) that accelerates the degradation but at a speed far less than expression.

This part can be used to construct the reporter for real-time invertase dynamics analysis.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without start code and restriction enzyme activity. This sequence is absolutely compatible with brick construction method and usually less likely to make trouble.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None