Reporter

Part:BBa_K1641024

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)
Revision as of 11:11, 10 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Reporter of Invertase activity of Cre, pInv-rep-100LoxM

This is the reporter of inversion activity of invertase Cre. An up-side-down mcherry gene with RBS is surrounded by a pair of LoxP and controlled by a constructive promoter BBa_J23101. At first stage, due to the meaningless mRNA, red signal cannot be generated, but when Cre exist,it inverts and restore the mcherry gene between two LoxP, the red signal can be generated at once.

This device is constructed to measure the real-time dynamics of timing module of Cre in the Micro-timer presented by SYSU-CHINA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None