Device

Part:BBa_K1742013

Designed by: Ruben Escriba Piera   Group: iGEM15_Valencia_UPV   (2015-09-10)
Revision as of 10:37, 10 September 2015 by Registry (Talk | contribs)

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35S:PhiC31:T35S - 35S:ReporterPhiC31::GFP:T35S

Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1449
    Illegal NheI site found at 1619
    Illegal NheI site found at 2661
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1107
    Illegal BamHI site found at 1844
    Illegal BamHI site found at 1874
    Illegal BamHI site found at 1960
    Illegal BamHI site found at 1970
    Illegal XhoI site found at 1801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1041
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2331
    Illegal SapI.rc site found at 2656


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None