Part:BBa_K1618028

Chloroplast Transit Peptide

This is synthetic a 5'UTR, omega (Tobacco Mosaic Virus. The chloroplast transit peptide provides subcellular localisation to the chloroplast.

NRP-UEA

At NRP-UEA, we used the chloroplast transit peptide in 8 of our composite constructs in order to make sure that our parts reached the chloroplast, where starch is produced. For 4 of these constructs, we included a Yellow Fluorescent Protein tag, in order to visualise if our parts did in fact reach the chloroplast. Using a confocal microscope, we were able to get the following images, in which the chloroplast is red and our protein with the fluorescent tag is yellow.