Composite

Part:BBa_K1675004

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-10)
Revision as of 07:33, 10 September 2015 by Registry (Talk | contribs)

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J23119+B0034+NhaA(BBa_K1675000)

The constitutive promoter J23119, the ribosome binding site B0034 and the functional gene NhaA were used in this new standard part. This part is available for the bacteria to survive in alkaline environment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 521
    Illegal BamHI site found at 1058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 384
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
biologyEscherichia coli str. K-12 substr.