Composite
ProGlgX

Part:BBa_K1618026

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-09-03)
Revision as of 14:42, 9 September 2015 by Cym13puu (Talk | contribs)

GlgX with IPTG-inducible promoter

GlgX is a genetic sequence which encodes for a glycogen debranching enzyme which will cleave alpha-1,6 linkages in glycogen, therefore reduce branching in glycogen to create a more linear molecule. GlgX is preceded by an IPTG-inducible promoter and both are encoded into the pSB1C3 standard vector. The bacteria are therefore chloramphenicol resistant.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None