Coding
ProGlgB

Part:BBa_K1618022

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-08-30)
Revision as of 14:31, 9 September 2015 by Cym13puu (Talk | contribs)

GlgB with IPTG-inducible promoter

GlgB is a genetic sequence which codes for a glycogen branching enzyme which adds branches onto glycogen strands. The enzyme cleabes alpha-1,4 linkages in glycogen strands and re-anneals the cleaved strand back onto the main glycogen strand through an alpha-1,6 linkage. This therefore creates the branch. The coding sequence is preceded by an IPTG-inducible promoter and both sequences are encoded within the pSB1C3 vector. The bacteria therefore shows chloramphenicol resistance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917


[edit]
Categories
Parameters
None