Coding

Part:BBa_K1773003:Design

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-06-09)
Revision as of 12:44, 9 September 2015 by Saras20 (Talk | contribs)

I-F type CRISPR-Cas Cas3 gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2623
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1344
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2307
    Illegal AgeI site found at 2680
  • 1000
    COMPATIBLE WITH RFC[1000]
(Restriction with EcoRI (E), XbaI (X) and PstI (P) of Wild type (WT) Cas3 gene compared to mutated Cas3 genes. The first mutant plasmid has two successful mutations, whereas the second mutant plasmid only has one successfully mutated restriction site.)

LALALALA

Design Notes

no


Source

A.actinomycetemcomitans D7-S

References