Coding

Part:BBa_K1675003:Design

Designed by: JIAQI XU   Group: iGEM15_BIT-China   (2015-09-09)
Revision as of 09:31, 9 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


glutamate decarboxylase A （GadA）


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 700
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of TTT to TTC Phe.(site:129bp)


Source

Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3666180&to=3667580&strand=true

References