Coding
Part:BBa_K1675003:Design
Designed by: JIAQI XU Group: iGEM15_BIT-China (2015-09-09)
glutamate decarboxylase A (GadA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 640
Illegal AgeI site found at 700 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation of TTT to TTC Phe.(site:129bp)
Source
Escherichia coli str. K-12 substr. Sequence found through Genbank http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.3?report=genbank&from=3666180&to=3667580&strand=true