Composite

Part:BBa_K1592018

Designed by: Shuyan Tang   Group: iGEM15_HUST-China   (2015-09-09)
Revision as of 08:51, 9 September 2015 by Registry (Talk | contribs)

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Pgal1+rox1+cyc1_terminator

Pgal1+rox1+CYC1_terminator is used to compose the logic reverse control. GAL1 promoter from Saccharomyces cerevisiae. 1 UAS site allows GAL4 binding domain to enhance the expression from this promoter. ROX1 in the downstream of pGal1 are expressed to inhibit the promoter of anb1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1012
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
    Illegal AgeI site found at 1224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1005
    Illegal BsaI.rc site found at 1055


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Categories
Parameters
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