RBS
RBS

Part:BBa_K1642004:Design

Designed by: Can Yin   Group: iGEM15_SJTU-BioX-Shanghai   (2015-08-15)
Revision as of 08:08, 9 September 2015 by EvaJiang (Talk | contribs)

SD sequence of cpcB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Ribosome binding sites(RBS) control protein expression level to a great extent. The PcpcG2 promoter does not have a typical Shine-Dalgarno (SD) sequence upstream of the start codon. Reported weak expression may be due to the lack of a SD-like sequence[], so we add an SD sequence on the gene under PcpcG2, hoping that the gene will express on a relatively high level.

Reported about 15-fold higher GFPuv-derived fluorescence intensity resulted from the addition of the SD-like sequence to PcpcG2 proved that the SD-like sequence function strongly in Synechocystis sp. PCC6803.


Source

RBS

References

Engineering of a green-light inducible gene expression system in Synechocystis sp. PCC6803