Coding
orfX

Part:BBa_K1668003:Design

Designed by: Xintian Xu   Group: iGEM15_ZJU-China   (2015-09-08)
Revision as of 17:46, 8 September 2015 by Registry (Talk | contribs)

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orfX (from Streptomyces avermitilis, increasing avermectin production)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 160
    Illegal NotI site found at 247
    Illegal NotI site found at 361
    Illegal NotI site found at 595
    Illegal NotI site found at 745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 510
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 463
    Illegal AgeI site found at 264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 441
    Illegal BsaI.rc site found at 195


Design Notes

We use seamless assembly.


Source

The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers metK1 and metK2

References