Coding
frr

Part:BBa_K1668002:Design

Designed by: Xintian Xu   Group: iGEM15_ZJU-China   (2015-09-08)
Revision as of 17:36, 8 September 2015 by Registry (Talk | contribs)

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frr (from Streptomyces avermitilis, increasing avermectin production)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 337


Design Notes

We used seamless assembly.


Source

The metK gene was amplified by PCR with genomic DNA of S. avermitilis(具体型号) as template using primers

References