Composite

Part:BBa_K1583108

Designed by: Stefan Robert Marsden   Group: iGEM15_TU_Delft   (2015-09-08)
Revision as of 10:52, 8 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP

Upon induction with rhamnose, a fusion protein of CsgA with a hydroxyapatite affinity tag is expressed. The CsgA protein monomers can aggregate to form amyloid nanofibres. The affinity tag is supposed to increase adhesive properties of the biofilm created as a result of the CsgA induction. In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/part_collection/2015
Parameters
None