Composite
Part:BBa_K1583108
Designed by: Stefan Robert Marsden Group: iGEM15_TU_Delft (2015-09-08)
pRha + CsgA with Hydroxyapatite-affinity tag & pTet + RFP
Upon induction with rhamnose, a fusion protein of CsgA with a hydroxyapatite affinity tag is expressed. The CsgA protein monomers can aggregate to form amyloid nanofibres. The affinity tag is supposed to increase adhesive properties of the biofilm created as a result of the CsgA induction. In order to better visualize the individual cells in the biofilm, we added the constitutive expression of RFP to our plasmid in a different operon. For our design, we took advantage of the double terminator present in the biobrick I13521 by placing it in front of our genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 635
Illegal AgeI site found at 747 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
//awards/part_collection/2015
None |