Composite
Part:BBa_K1660008
Designed by: Dai Yuanyi Group: iGEM15_BNU-CHINA (2015-09-08)
J23100-B0034-bace16
This part encodes a serine protease in Bacillus nematocida target essential intestinal proteins and kill nematode under the control of a constitutive promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 56
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 152
Illegal NgoMIV site found at 515
Illegal NgoMIV site found at 587
Illegal NgoMIV site found at 839
Illegal AgeI site found at 446 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme
chassis | E. coli BL21(DE3) |
function | virulent to nematode |
origin | Bacillus nematocida B16 |
resistance | Chloramphenicol |