Composite

Part:BBa_K1660008

Designed by: Dai Yuanyi   Group: iGEM15_BNU-CHINA   (2015-09-08)
Revision as of 07:01, 8 September 2015 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23100-B0034-bace16

This part encodes a serine protease in Bacillus nematocida target essential intestinal proteins and kill nematode under the control of a constitutive promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 56
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 152
    Illegal NgoMIV site found at 515
    Illegal NgoMIV site found at 587
    Illegal NgoMIV site found at 839
    Illegal AgeI site found at 446
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
chassisE. coli BL21(DE3)
functionvirulent to nematode
originBacillus nematocida B16
resistanceChloramphenicol