DNA

Part:BBa_K1732004:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-07)
Revision as of 03:20, 8 September 2015 by Donnalee (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
    Illegal BamHI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715


Design Notes

J23100 constitutive promoter (BBa_J23100), a RBS (BBa_B0034), CDcel domain (BBa_K1732002), Gaussia luciferase (BBa_K1732003), and B0015 terminator (BBa_B0015). A 6X His-tag was added for easy purification of the proteins.

E.coli was codon optimized for E.coli.

Source

Synthesized using IDT by Cheryl Telmer from the Bruchez lab in Carnegie Mellon University.

References