DNA

Part:BBa_K1732004:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-07)
Revision as of 03:18, 8 September 2015 by Donnalee (Talk | contribs) (→‎Source)


pSB1C3-J23100-B0034-CDcel-Gaussia-His-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1517
    Illegal BamHI site found at 975
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 715


Design Notes

E.coli was codon optimized for E.coli.


Source

Synthesized using IDT. Sequence was obtained from Cheryl Telmer from the Bruchez lab in Carnegie Mellon University.

References