Device

Part:BBa_K1742012

Designed by: Mónica Victoria Gutiérrez Salazar   Group: iGEM15_Valencia_UPV   (2015-09-07)
Revision as of 15:30, 7 September 2015 by Registry (Talk | contribs)

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35S:Gal4BD-DronpaK:T35S - 35S:DronpaN-VP16:T35S

Translational unit composed by the promoter 35S, the T35s terminator, the CDS Dronpa(145K), Dronpa(145N), the binding domain Gal4 and VP16. The CDS works as a toggle switch activated with green light and deactivated with blue light. Dronpa(145K) changes its conformation with light, being able to dimerize with Dronpa(145N), acting as a transcription factor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3335
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 392
    Illegal BglII site found at 1138
    Illegal XhoI site found at 1342
    Illegal XhoI site found at 1655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1574


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None