Composite
SV40+Rluc

Part:BBa_K1722013:Design

Designed by: Hao Wang   Group: iGEM15_SZU_China   (2015-09-07)
Revision as of 12:27, 7 September 2015 by Registry (Talk | contribs)

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SV40+Rluc Composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 897


Design Notes

We designed the primer of Rluc and amplified it from the vector psi-Check2, the Rluc gene was then flanked by the iGEM prefix and suffix after amplification. We constructed the two genes in pSB1C3 using 3A Assembly as described in iGEM.org.


Source

SV40 promoter is achieved from 2015 Distribution Kit(BBa_K1150011). Rluc is achieved from Shenzhen Second People's Hospital.


References