Device
Part:BBa_K1742009
Designed by: Pilar Baldominos Group: iGEM15_Valencia_UPV (2015-09-07)
35S:BxbI:T35S - 35S:ReporterBxBI::GFP:T35S
Composite composed of the recombinase Bxb1 optimize for Nicothiana Benthamiana and a reporter to prove its action. The reporter composite consist in a terminator flankeated by the recognition sequence of Bxb1 between the promoter and the CDS. Only if the recombinase is present, the GFP will be observed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1959
Illegal BamHI site found at 1141
Illegal XhoI site found at 660
Illegal XhoI site found at 1146 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 49
Illegal NgoMIV site found at 1460
Illegal NgoMIV site found at 1698 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2170
[edit]
Categories
Parameters
//chassis/eukaryote/nbenthamiana
None |