DNA

Part:BBa_K1636002

Designed by: Diana Montaño, Alberto Cristerna, Jessica Avila, Ernesto Hernandez, Camila Martinez, Fernanda Sanchez, Roger Milton Rubio Sanchez, Ana Laura Torres   Group: iGEM15_TecCEM   (2015-09-05)
Revision as of 01:07, 6 September 2015 by Registry (Talk | contribs)

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Linker

This linker was designed by the iGEM TecCEM team in order to retrieve single-stranded oligonucleotides when produced as single-stranded genetic material (e.g. plasmid). The linker should be flanking said oligonucleotides, and given the design of its sequence, it is able to form a hairpin-like secondary structure that allows the usage of a restriction enzyme (MlyI from New England BIolabs) with a blunt cut thereby releasing the oligos of interest.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None