Coding

Part:BBa_K1699002:Design

Designed by: Emil Ruvinov   Group: iGEM15_BGU_Israel   (2015-08-09)
Revision as of 19:30, 5 September 2015 by Shalevgo (Talk | contribs) (→‎References)

gRNA for SaCas9 targeting human ubiquitin B gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 146
    Illegal NgoMIV site found at 175
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Cloned into pSB1C3 using EcoRI and PstI restriction sites.
F. tagtcatgGAATTCGCGGCCGCTTCTAG
R. tgtatactCTGCAGCGGCCGCTACTAG

Source

IDT de-novo synthesis.

References

1. In vivo genome editing using Staphylococcus aureus Cas9.
10.1038/nature14299

2. Self-processing of ribozyme-flanked RNAs into guide RNAs in vitro and in vivo for CRISPR-mediated genome editing
http://onlinelibrary.wiley.com/doi/10.1111/jipb.12152/full