Part:BBa_K1824562
RNA Thermometer U6 (Specially designed for J23119)
This is a specially designed U6 RNA thermometer that with a unique spacer at the front, which would make it compatible with promoter BBa_J23119.
U6 RNA thermometer have the hairpin structure that harbors the Shine-Dalgarno sequence (SD sequence) and, in this way, make it inaccessible to the 30S unit of the bacterial ribosome, resulting in translational inactivation (Figure 2). The melting temperature of this RNA thermometer is 37 Celsius degree. Once reaching the melting temperature, hairpin structure would vanish and as a result, exposing the SD sequence to trigger the translation process.
Different promoters have their own transcription start sites and, in most cases, + 1 sites are embedded in promoter sequence. Hence, it is normal that transcribed RNA usually carry part of promoter sequence. However, for regulatory parts like RNA thermometer, truncation or alteration of the RNA sequence could be destructive. Hence, special designed RNA spacer between transcribed part of promoters and RNA thermometers are important for maintaining the secondary structure of RNA thermometer.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 25
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 25
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 11
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 25
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 25
- 1000COMPATIBLE WITH RFC[1000]
| None |