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Part:BBa_K1766001

Designed by: Sarah Wideman   Group: iGEM15_Stockholm   (2015-09-05)
Revision as of 15:19, 5 September 2015 by Sarahwideman (Talk | contribs)

MicF Target


The micF target stems from the 5’ untranslated region of the ompF (outer membrane porin F) gene in E. coli. It is the bind site for micF, a non-coding regulatory RNA. MicF prevents translation of ompF when it binds to the micF target [1].

BBa_K1766001 consists of the micF target under the control of Anderson promoter BBa_J23101.

The micF target can be fused to a protein of interest. It is then possible to down-regulate protein expression using MicF (BBa_K176600).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] Delihas, N; Forst S (2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". J Mol Biol 313 (1): 1–12


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