Coding

Part:BBa_K1595000:Design

Designed by: Jonlin Chen   Group: iGEM15_Cornell   (2015-08-24)
Revision as of 08:05, 5 September 2015 by J.Chen (Talk | contribs) (→‎Design Notes)


EcnB from Ralstonia pickettii


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 123
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 123
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 123
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use in conjunction with strain-expressing T7 polymerase

Has internal PSTI cutsite.

Source

Synthesized by Integrated DNA Technology

References