Coding

Part:BBa_K1742001:Design

Designed by: Ruben Escriba Piera   Group: iGEM15_Valencia_UPV   (2015-09-03)
Revision as of 02:03, 4 September 2015 by Registry (Talk | contribs)

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Entertoxin LTB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The protein consists of the E. coli enterotoxin beta subunit. It is codon optimized for plants and contains a C-terminus 6X Hys tag in order to detected by anti-Hys antibodies.


Source

The LTB protein was designed based on a publication that showed that it can be expressed in Nicotiana Benthamiana plants.

References