Coding
AsLOV2pep

Part:BBa_K1742000

Designed by: Ruben Escriba Piera   Group: iGEM15_Valencia_UPV   (2015-09-03)
Revision as of 01:35, 4 September 2015 by Registry (Talk | contribs)

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Avena sativa LOV2 domain

Plants and bacteria contain a variety of photoreceptors that activate intracellular signalling in response ti light. Among these, the most extensively studied are LOV (light/oxygen/voltage) domains. On blue light (peak 450nm) aabsorption, the FMN cofactor in the LOV domain forms a covalent bond with a cysteine residue. In the LOV2 domain of plant phototropin, the FMN-cysteine bond formation leads to partial unfolding of the C-terminal alpha-helix from the rest of the LOV2 domain. Strickland et al. created LOV2 fusions capable of light-mediated heterodimerization by fusing the LOV2 domain to peptides that bind to PDZ domains.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/nbenthamiana
Parameters
None